Phosphoporin
WebOct 1, 2002 · LPS-free Phosphoporin PhoE from E. coli— The mature form of PhoE protein was expressed and isolated from E. coli strain Bl21(DE3) cells containing plasmid pCJ2 and refolded into a trypsin ... WebPHOSPHOPORIN. NCBI. National Center for Biotechnology Information. Search. 1PHO: Crystal Structures Explain Functional Properties Of Two E. Coli Porins. PDB ID: 1PHO Download: MMDB ID: 57112: PDB Deposition Date: 1993/1/15: Updated in MMDB: 2012/10: Experimental Method: x-ray diffraction.
Phosphoporin
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Porins form aqueous channels that aid the diffusion of small hydrophilic molecules across the outer membrane of Gram-negative bacteria. The crystal structures of matrix porin and phosphoporin both reveal trimers of identical subunits, each subunit consisting of a 16-stranded anti-parallel beta-barrel containing a pore. WebThe phosphoporin PhoE is synthesized when cells are grown under phosphate limitation ( 5 ). PhoE has a molecular weight of Mr 36,822 and an exclusion size of Mr ∼600 and is weakly anion selective ( 6 ). The crystal …
WebSep 1, 2009 · VCA1008, an uncharacterized OMP involved in V. cholerae pathogenicity, presents sequence similarity to porins of Gram-negative bacteria such as phosphoporin PhoE from Escherichia coli. A three-dimensional model shows that VCA1008 is a 16-stranded pore-forming beta-barrel protein that shares three of the four conserved lysine … Webphosphoporin PhoE 64296871 - Gene ResultphoE phosphoporin PhoE [] Gene provides a unified query environment for genes defined by sequence and/or in NCBI's Map Viewer.
WebDec 20, 1991 · Phosphoporin is a pore-forming transmembrane protein that spans the outer membrane of Escherichia coli and facilitates the diffusion of phosphates and … WebThe putative phosphoporin encoded by vca1008 of Vibrio cholerae O1 is expressed in vivo during infection and is essential for the intestinal colonization of infant mice. In vitro, its …
WebThe use of natural systems, such as outer membrane protein A (OmpA), phosphoporin E (PhoE), ice nucleation protein (INP), etc., has been proved very useful for the surface exposure of proteins on the outer membrane of Gram-negative bacteria.
WebDec 20, 1991 · Phosphoporin has been crystallized in several different crystal forms, although only one appears to be suitable for X-ray analysis. These crystals, which are hexagonal plates, diffract X-rays to 3 Å resolution and belong to the space-group P 6. Keywords crystallization membrane protein porin ion channel X-ray crystallography scotch 2 blank tapesWebPorin is a homotrimeric molecule, which forms a pore in the outer membrane, and probably functions as an ion channel. As a major surface protein that is constitutively expressed in all strains and one of the least variable, it has been proposed as a vaccine antigen (see below). scotch 309snWebVCA1008, an uncharacterized OMP involved in V. cholerae pathogenicity, presents sequence similarity to porins of Gram-negative bacteria such as phosphoporin PhoE from … scotch 2 way tapeWebAug 27, 1992 · Porins form aqueous channels that aid the diffusion of small hydrophilic molecules across the outer membrane of Gram-negative bacteria. The crystal structures of matrix porin and phosphoporin both reveal trimers of identical subunits, each subunit consisting of a 16-stranded anti-parallel beta-barrel containing a pore. scotch 3028WebOct 1, 1991 · Four new crystal packings of Escherichia coli porins are presented (phosphoporin, maltoporin, and two crystal forms of matrix porin). These were determined by molecular replacement methods using a polyalanine trial model acquired from the refined coordinates of porin from Rhodobacter capsulatus.The successful molecular replacement … scotch 3071Webphos·pho·phor·in ( fos'fō-fōr'in ), A protein (MW 155,000) found in dentin that is believed to have a role in mineralization. Farlex Partner Medical Dictionary © Farlex 2012 Want to … scotch 2 sided permanent tapeWebDec 20, 1991 · Phosphoporin is a pore-forming transmembrane protein that spans the outer membrane of Escherichia coli and facilitates the diffusion of phosphates and … scotch 3082 tape