WebApr 6, 2024 · Blood was diluted with PBS and PBMCs were obtained by density gradient centrifugation using Ficoll solution (density of 1.077, Biocoll Separating Solution, Biochrom AG, Berlin, Germany). PBMCs (10 6 /mL) from healthy dogs were added to cells infected with rNDV in 24-well plates. PBMCs were collected and suspended in RLT buffer … WebPBS and permeabilised for 25 min with 500 μl 0.5% Triton/ PBS. After washing with PBS, non-specific protein binding sites were blocked with 500 μl 10% FCS/PBS for 1.5 h. Cells were washed with PBS and incubated for 1 h with 100 μl rabbit anti Ki67 antibody (1:100 in 1% FCS/PBS). After wash-es with PBS, the cells were incubated for 1 h with ...
The Biochrom 30 series of Amino Acid Analysers - Potencial …
WebPbs, supplied by Biochrom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebThe PBS solution (cat. no. L1815) is adjusted to a pH of 3.0 - 3.5 as follows: Add 1 ml 1 N HCl to 100 ml PBS solution. Add 2 ml of the diluted Collagen solution per 10 cm2 area of … how much money is a radiant steelix
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